Impact of Training and Developement on Employees Essay Example for Free

Impact of Training and Developement on Employees Essay 1.1 Introduction The purpose of training is to help people learn something they need to know or able to do for specific purpose such as to achieve organizational objectives and goals, carry out specific tasks, prepare new responsibilities or attain their correct goals. Successful training programs share they affect changes, that are designed to achieve objectives that describe what people will be able to do as result of training are learned centered, not trained centered. According to paymond A. Noe etal, 2011: p 7 training is a planed effort to enable employees to learn job related knowledge, skills and behavior. For example many organizations offer safety training to teach employees work safety habits . development involves acquiring knowledge, skill and behavior that improve employees ability to meet the challenges of a variety of new or existing jobs, including client and customer demands of those jobs (Ibid). Development programs often focus on preparing employees for management responsibility, like wise if company plans to set up teams to manufacture products , it may offer development program to help employee learn the ins and outs of effective team work. Development implies â€Å"learning that is not necessary to related to employees current job† and development indicates that it is future oriented (M. londom 1989) training has the distinct role in achievement of an organizational goal by incorporating the interests of organization and the work force( stone R.j Human resource management, 2002) . however Training and development focus on enabling the employees to perform their duties better While HR functions focus on the day to day operations of the company, such as payroll, disability, and employee and management conflicts or issues. HR protects employees and the company by following the correct and legal guidelines for resolutions. 1.2 back ground of organization In 1994 STC (Somaliland Telecommunication Corporation) established by one of Somalilander and it was first Telecommunication Company in Somaliland. Later company started to open share by public in order to maximize capacity of the company. Then company expand service throughout main cities of Somaliland, such as Berbera Burco, Boorama and Lowyocado, then it become the biggest Telecommunication Company in Somaliland at that time It was booming for several years then conflict between partner the main source of the conflict was the administration. Which makes difficult the company to be competitive position compare to other that is why liquidation comes too finally. In 2006 January group of Somalilander, and Somalis bought the whole company they change the name of the company, from STC to SITALINK under the name of SITALINK it was working couple years. In 2008 JULY the name charge once more in to NATIONLINK, which is right now legal name of the company. This is Telecommunication Company that offering Service is All the Somaliland, Somali and other countries like central Africa, Congo, and Switzerland and so on. This is Company corporate. Services offering This Company like: * Mobile Service (GSM) * Landlines (PSTN) * Internet (ISP) * E- Mail, Cash Payment (Mobile Money) * VoIP Service Vision Working toward the future demands that we not only focus on todays needs, but also develop technologies for the needs of tomorrow. Mission Change and dynamic thinking is a distinguishing feature we relished over the years. In order To keep up with the pace of innovation and growth in the business of telecommunication our only choice is to embrace forward thinking and better adaptation to change. To perfect the art of better adaptation to changes is not a matter of choice, but out of sheer necessity. 1.3 statement of problem My preliminary indirect observation shows us that there is no specific policy of training and development that is guided to employees. And also invested employees with high cost of training which are sometimes sent to overseas, move from the organization, hence this is loss and it affects the efficiency of an organization. but on the other hand Nation link has all mostly HR policies that is written on organizational procedures, which are delivered to employees in order to act on their activities. As human resource officer of Nation link told the researcher that their HR policies based on Somaliland labor law. 1.3 Research questions 1.4.1 Did they have HR policy? If yes what did their policy state training and development program conducted in their organization? 1.4.2 Is there any criterion to select trainees? 1.4.3 What is training methods used? 1.4.4 Did training and development program have impact on organization’s performance? 1.4.5 Who do they use to do their training; ? 1.4 objective of the study 1.5.6 general objectives of the study is to assess the impact of training and development on employees performance of Nation link 1.5.7 The specific objective are :- a.) To establish HR policy especially training and development policy b.) To describe how they select trainees   c.) To know training method(s) used to conducting training. d.) To understand that training and development have impact on performance of an organization. 1.5 scope of the study Since it is difficult to comment on each and every department of nation link due to both the researcher and organization’s respondents busy as well and limited time, for these barriers, the researcher would like to focus only on the HR department at the Headquarter in Hargeisa town, because HR activities are handled mainly at the headquarter and this limits the study on activities of training and development. 1.6 significance of the study

The Major Components Of Green Computing Information Technology Essay

The Major Components Of Green Computing Information Technology Essay As IT has become a major component in social infrastructure, it plays a vital role in humans life. Computing is a fast growing area which consumes energy a lot. A computer which performs well consumes lots of energy. It takes a lot of energy to create, package and retrieve data. With the development of the technologies, people tend to depend on these technologies more and more. It will lead us to many problems as energy is a non renewable resource. It will soon cost more to power a computer in future, than buying a new one today. Therefore we have to find methods, where we can minimize the wastage of energy while utilizing existing resources efficiently. Since computer plays a major role in our lives, demand for the energy, cost and wastage also increase dramatically. According to the Climate Saving Computing Initiative, the half of the power input to our PC is wasted without even reaching to the processor, memory, disk or any other component. Further, they have identified that added heat of the computer systems, inactive sleep and hibernate state of the computer and mal practices of using electronic equipments has a great impact in this energy wastage. It is estimated that out of $250 billion per year spent on powering computers worldwide only about 15% of that power is spent computing. The rest is wasted idling. Therefore, Green Computing is introduced in order to maximize the energy efficiency and increase the recyclability of products and the factory waste. Green Computing is focusing on creating technologies that help to preserve nature and minimize the bad effects of the technological development. Overview Green Computing is an echo friendly approach to the modern computing environment. It discusses about the ways of using computers in environmental friendly way. Â  The main focus of Green computing is reducing the environmental impact of industrial processes and innovative technologies caused by the Earths growing population and the ways of utilizing computer resources efficiently. It talks about serving social needs in a way which reduce the damage happen to the nature. Mainly this is talking about creating recyclable products, reducing pollution, providing alternatives to certain technological areas and etc. It is a concept which is already used in the IT environment. Researchers have found that most of the computers are not used for most of the time, but they are left running and many personal computers are left on unnecessarily. It leads to the high wastage of energy. These kinds of issues are also considered in Green Computing. It also talks about proper disposal of electronic w aste. Eco Friendly Computing Green Computing is also known as Green IT. Environmental sustainability, Energy efficiency, and the cost of disposal and recycling also includes in Green IT. History The Energy Star program commenced by the U.S. Environmental Protection Agency in 1992 was the first voluntary labelling program designed to promote and recognize energy efficiency. It is basically designed to promote and recognize energy efficiency in monitors, climate control equipments and other technologies. The term Green Computing immerged shortly after this Energy Star Program. The first function launched as a result of the Green Computing researches was the sleep mode function of computer monitor. Approaches to Green Computing In order to gain the environmental sustainability and efficient use of energy through computing there are four main paths to be taken. Green Use Using the computers and other related products in an efficient manner where the energy consumption is minimized. Green Disposal Reusing old computers, properly disposing and recycling other unwanted products. Green Design Designing energy efficient and environmentally friendly computers and accessories. Green Manufacturing Manufacturing computers and other related equipments in a way that they have a minimal effect to the environment. These four steps can be further spanned in to number of activities and areas. Efficient use of energy Power saving Server virtualization Environmental sustainable designing Responsible disposal and recycle Risk mitigation Use of renewable energy sources Eco- labelling Use of Green methodologies and assessment tools. As an initial step to the Green Computing the work habits of the individual computer users have to be changed. Most of the energy wastages regarding the personal computer usage happen because of the false beliefs regarding the computer usage. For an instance, people belief that when turning off a computer and turning on it back uses more energy than it leaving on. But the actual energy consumption of the computer when it boots up, is far less than the energy it uses when left on for more than three minutes. Further the use of notebook computers rather than using personal computers will also save energy. Moreover, people think that screen savers do save energy. But the fact is that they are designed to increase the lifetime of monochrome monitors. The screen savers will save energy if they actually turn off the screen or turn off the backlight of the laptops. By letting the users get to know about these facts will change their habits and it will lead to power utilization. When it comes to organizational level, many organizations have undertaken various kinds of activities as an approach to this Green IT. Telecommuting Virtualization VoIP Organizations have allowed their workers to work while being at home. This will help to reduce fuel emission at transportation. They are given all the necessary facilities to work. This is known as telecommuting. Virtualization refers to the consolidation of servers. It allows to two or more logical computer systems to run in one physical hardware. This leads minimum power consumption and maximum cooling. VoIP or Voice over Internet Protocol is a technology of voice transmission over internet or other packet switched networks. It reduces the telephone wiring infrastructure and is low cost. It is method of reducing metallic waste. Green business practices Green IT isnt just about energy efficiencies but also about operational efficiencies that can improve the organization. In most of the organizations, Green IT is practiced because of the explicit business drivers such as cost and the availability of the energy, cost of the equipments, cost of the data centres, business process optimization and performance and efficiency. When focusing on business optimization, few areas can be targeted which energy demand can be reduced and growth can be increased with the help of IT. Server and storage utilization and consolidation Server, storage, and application virtualization Technology and information life cycle strategies Energy measurement and management Green Information Strategy Under Green Information Strategy, the ways of managing and retaining information has been defined. The ways of collecting, classifying and archiving information are introduced in Green Information Strategy. It involves several key steps: Understanding the requirements for information retention and availability. Determining infrastructure requirements. Conducting continual strategic planning to meet economic and business conditions and demand. Measuring progress and adjusting the strategies. Information Lifecycle Management is a set of concepts which helps organizations to build processes and implement best practices for creating, storing, archiving, and dispose data. A variety of technologies and methodologies can be used in order to optimize the storage utilization. Then the amount of storage required and the energy used to power will be reduced. Maximizing data centre and server room efficiency Designing a data centre for maximum efficiency is complex and requires input from many disciplines not normally associated with IT. But most of the organizations dont have data centres. They have their IT equipments in a sever room. Server room is a data centre operated in a small scale. Here are few facts that have to be considered when designing a server room. Raised floor in computer rooms was the general accepted norm since vacuum tube era. Variable speed motors can tailor cooling power usage to need, producing substantial savings, and may prove less expensive in the long run though they are more expensive to purchase. The trend in servers is to pack more CPUs in to a single track. But when power densities get above 200 watts per square foot, because of the high heat generated, too much air is needed to remove the heat. Therefore racks (19-inch EIA-310-D standard) with built-in chillers or even water-cooled servers, become preferable. Use a large server. Replace physical servers with virtual servers. Blade servers Blade servers put each processor, memory and hard drive on one single card called blade. These blades are fixed in to a backplane. A single power supply, a shared cooling system, a shared network interface is used among the blades, which connected to a backplane. Replacing multiple servers with blade servers generally saves power and space. Green Storage Storage systems are among the systems which consume power and storage a lot. Drivers have to be full powered and running to be able to store data. Data Deduplication Data Deduplication is the term used for removing the duplicates. It examines the hard drive or the storage system and find out the duplicates and remove them. It effectively reduces the storage allocated for certain data file and allocates it to other additional data. It increases the capacity of your system. Thin Provisioning Thin provisioning is allocating right amount of storage is as needed. It helps to eliminate unused capacity. Storage can be used efficiently by allocating the required storage when it is needed and using storage management software to adjust it. As the storage is dynamically allocated the storage can be grown or shrink according to the application stored. Further storage management software can view applications simultaneously and allocate unused capacity as needed. Storage Virtualization Storage virtualization gets all the available capacity together from multiple storage systems and enables it to share, without requiring a change in storage hardware. By enabling sharing it avoids the trapped capacity, which is available but cannot be used by an application. Using Virtualization Multiple applications and multiple Operating systems can be run simultaneously with the use of virtual memory technology. Virtualization increases the utilization of computer systems by condensing applications into few servers. Therefore the number of required servers will be reduced. It reduced the power consumption of the data centres and the cooling requirements. Virtualization is one of the earliest paths to green, because when more than one application is run on a server (application consolidation) the number of servers required to support these applications is reduced. Fewer servers mean less power consumption and low energy requirement for cooling. Future Directions on Green Computing

Dissection of Microrna-30Ds Function Roles

Dissection of Microrna-30Ds Function Roles DISSECTION OF MICRORNA-30DS FUNCTION ROLES IN MAMMALIAN PANCREATIC-BETA CELLS By:  Yiping Mao   ABSTRACT MicroRNAs (miRNAs) are a group of small non-coding RNAs (about 21-22 nucleotides long) that fine tune target protein output through messenger RNA degradation or inhibition of its translation. Recent studies showing that miRNAs and their function components respond to cellular stress to maintain steady state physiology of the cells. Since there are thousands of microRNAs existing in all kind of cells, their functional characterization during the normal states or stress conditions is not fully addressed yet. In this thesis, important aspects of pancreatic ÃŽÂ ²-cell function under normal or stress condition such as apoptosis, proliferation, insulin production and release and their regulation by the miRNA were explored. Pancreatic ÃŽÂ ²-cell is a group of insulin producing cells and plays critical role in maintaining glucose hemostasis. By combining mouse and cell line genetic approaches, high-throughput deep sequencing, a list of cell assays and molecular techniques, we have shed light on the novel roles of miR-30d, one highly expressed miRNA when ÃŽÂ ²-cell responding to high glucose stimulus, in regulating ÃŽÂ ²-cell mass on the middle aged mice. We demonstrated that overexpress of miR-30d deteriorated glucose tolerance ability of the mice with or without high fat diet treatment by significantly reducing the ÃŽÂ ²-cell mass with less insulin production. Additionally, we demonstrated that the reduced ÃŽÂ ²-cell mass is because both apoptosis pathway and proliferation p athway have been effected by miR-30d by targeting variety of protein factors expression. BCL2 interacting protein 3 (BNIP3) and cyclin E2 (CCNE2) have been respectively confirmed as miR-30ds targets and the effect of their regulation by miR-30d in pancreatic ÃŽÂ ²-cell proliferation or apoptosis aspects have been addressed as well. Furthermore, we could show that silencing of miR-30d in MIN6 cell (ÃŽÂ ²-cell mimicking cell line) by CRISPR-CAS9 gene editing system promotes the insulin secretion, which is through potentiated expression of MAFA, an insulin transcription factor. These studies uncovered novel functional roles of the miR-30d pathway in mediating ÃŽÂ ²-cell function and fate. Further dissection of these pathways shall uncover several mechanisms by which the ÃŽÂ ²-cells undertake to maximize their efficiency during disease states such as T2D.   CHAPTER 1: INTRODUCTION AND BACKGROUND 1.1 The Brief History of Diabetes and Research Long before terming diabetes, which means to pass through by Greek Apollonius of Memphis in 250 BCE and the re-discovery of honey-like-urine (glycosuria) by Thomas Willis, who included the term mellitus, Diabetes has discovered its first saying around 1500 B.C. in an Egyptian manuscript. It was perceived as a disease related with too great emptying of the urine (polyuria). Later, important discoveries includes Matthew Dobsons first proof of elevated urine and blood glucose levels (hyperglycemia) in people with diabetes (Dobson, 1776). In 1889, Joseph von Mering and Oskar Minkowski were the first to give evidence that pancreas removal in dogs induced diabetes, proposing that pancreas functional related to glucose levels. Afterward, Edward Albert Sharpey-Schafer proposed that diabetes could be brought on by losing a pancreatic chemical, which he named as insulin in 1910 (Polonsky, 2012). Taken together, diabetes is currently well recognized as a gathering of heterogeneous disorders cha racterized by hyperglycemia because of loss of insulin or its effectiveness. Current worldwide trends demonstrate a surprising 382 million individuals have diabetes and this is predicted to ascend to 592 million by the year 2035 (IDF Diabetes Atlas, 2013). The cases of diabetes complications, including diabetic retinopathy, cardiovascular disease and renal failure are constantly rising and the death rate because of those are worsen every year. Claude Bernards identification of liver as the major glucose production organ led to the first concept of homeostasis which has been termed and expanded by Walter Bradford in the mid-nineteenth century, to describe the maintaining of steady-state physiology of the cells (Robin, 1979). This gave the notion that actually the disturbed glucose homeostasis is one of the important events of the diabetes progression. Given the evidences of insulin is involved in maintaining the glucose homeostasis, Frederick Banting and Charles Best integrated a series of scientific approaches, and were able to purify the insulin from the pancreas. Moreover, they successfully treated the patients who suffer from the diabetes, with their purified insulin (Banting Best, 1922; Banting et al., 1922). This landmark finding set the stage for treating the severe diabetes with insulin. However, it has been almost a century now since the first time insulin was discovered and purified, diabetes remains the incurabl e disease, requires life-time attention and treatment because of its complexity. Diabetes has been classified as a couple different types nowadays, the major two types are known as the Type I Diabetes (T1D) or Insulin Dependent Diabetes Mellitus (IDDM) and Type II Diabetes (T2D) or Non-Insulin Dependent Diabetes Mellitus (NIDDM). Surprisingly, these two major types has been notified as early as 100-200 B.C by Indian physicians (Kahn, 1994). However, the first scientific evidence was brought by Wilhelm Falta and Harold Himsworth after they set the standardized glucose/insulin tolerance test in human to distinguish the insulin sensitive from non-sensitive patients (Himsworth, 1936). Insulin dependent T1D is featured in insufficient insulin production due to autoimmune response to the pancreatic beta-cells and it mostly affects young kids. T2D is more described as a metabolic syndrome with emergence of insulin resistance and obesity etc, and it is more common among adults (Moller, 2001). There is a complex network of several insulin responding tissues contributes to the maintenance of glucose homeostasis, and of course any abnormality in this network induces the progression of T2D. Besides the metabolic relevant tissues, the gene-interactions also play critical roles in the development of T2D and obesity such kind of metabolic diseases (Doria et al., 2008). 1.2 The Islet Architecture, ÃŽÂ ²-cell Fate determination The islets of Langerhans are specialized endocrine part of pancreas and are the only part producing the secreting hormones. They basically comprise of various cell sorts named ÃŽÂ ±, ÃŽÂ ², ÃŽÂ ´, PP, and ÃŽÂ µ that secret the islet hormones glucagon, insulin, somatostatin, polypeptide Y, and ghrelin individually, and these hormones are required to maintain the glucose homeostasis at normal or stress state. Furthermore, the islets are known to have dynamic and plastic architecture that is proposed to be adjusted over the time of development (Steiner et al., 2010). During the progression of insulin resistance, pregnancy or T2D, the islets extend in size to make up for expanded insulin requirement (Weir and Bonner-Weir, 2004; Kim et al., 2009). But afterwards, there is a considerable loss of ÃŽÂ ²-cell mass because of environmental or genetic factors, inducing the serious hyperglycemia in cause of insulin deficiency. While islets count to just ~1-2% of the whole pancreas, the insulin producing ÃŽÂ ²-cells represent ~65-80% of the islet mass, constituting to roughly 2% of pancreatic weight. Furthermore, the rest other cell types are considered as non ÃŽÂ ²-cells of the islets (Weir and Bonner-Weir, 2013). During the development, these distinctive cell types are known to emerge from a single progenitor cell that producing Neurogenin3 or Ngn3, a transcription factor that decides endocrine cell destiny (Edlund, 2002). Afterwards, other transcription factors, for example, Pdx1, Pax4, Nkx2.2, Nkx6.1, MafA, and Foxo1 help the ÃŽÂ ²-cell fate determination (Ziv et al., 2013). While it had been learned as that ÃŽÂ ²-cells proliferate by self-duplication from old ÃŽÂ ²-cells instead of differentiation from stem cell (Dor et al., 2004), some other study suggested that multipotent cells inside the pancreas could differentiate into ÃŽÂ ²-cells as well (Xu et al., 2008). The latter study is a lso confirmed by researches showing how expressing the ÃŽÂ ²-cell specific transcription factors in non ÃŽÂ ²-cell could trigger a ÃŽÂ ²-cell lineage in mice (Collombat et al., 2009; Al-Hasani et al., 2013). Recent studies additionally showing that non-ÃŽÂ ² cells, for example, ÃŽÂ ±-and ÃŽÂ ´-cells could experience transdifferentiation into ÃŽÂ ²-like cells when the mice is suffering from significant ÃŽÂ ²-cell loss (Thorel et al., 2010; Chera et al., 2014). On the other hand, a few researches demonstrate that the ÃŽÂ ²-cells can likewise lose their fate or dedifferentiate into non-ÃŽÂ ² or progenitor cells when losing any of the previously mentioned ÃŽÂ ²-cell particular transcription factor (Ziv et al., 2013). Moreover, a current study demonstrated that human ÃŽÂ ²-cells are capable of converting into ÃŽÂ ±-cells with no genetic modification (Spijker et al., 2013). Taken together, all these researches have showed that the dynamic plasticity of islet cells. 1.3 Glucose Stimulate Insulin Secretion One unique and significant feature of ÃŽÂ ²-cells is to detect the blood glucose changes and secrete insulin into extracellular milieu in response to keeping the glucose levels within the range of 4-8mM (Weir and Bonner-Weir, 2013). This is primarily accomplished by the take-up of extracellular glucose by the glucose transporter 2 (Glut2; Bell et al., 1990) at the plasma membrane. Upon uptake, the intracellular glucose sensor, glucokinase (Gck), subjects glucose moieties to quick metabolism system by glycolysis (Matschinsky and Ellerman, 1968). This brings about the producing of three carbon products: pyruvate, which takes part in the tricarboxylic corrosive (TCA) cycle inside the mitochondrion to eventually create adenosine triphosphates (ATP) by means of the electron transport chain system. The ATP therefore leads to the increase of ATP/ADP proportion in the cytoplasm, activating the closure of the ATP sensitive potassium (KATP) channel. Vitally, mutation in the kir6.2 subunit o f this channel were demonstrated to induce neonatal diabetes in both mice and human because of loss of insulin secretion as a consequence of constitutively open KATP channel (Koster et al., 2000; Gloyn et al., 2004). It has long been realized that glucose stimulates the closure of these KATP channels thus leading the slow menbrane depolarization (Ashcroft et al., 1984). This promotes extracellular calcium influx by voltage dependent calcium channels and potentiates the releasing of insulin (Matschinsky et al., 1998). Insulin is secreted in an oscillatory manner because of the blood glucose level and triggers downstream insulin signaling cascade in insulin-responsive tissues for the taking up glucose. It has well been shown that islets can be entrained to small changes in glucose and thus the plasma insulin has high frequency of oscillation. However, this capacity of entrainment of the islets is disturbed in patients with T2D (Mao et al., 1999). It exhibits ÃŽÂ ²-cell malfunction because of loss of insulin secretion is a major issue during the clinical indication of T2D. 1.4 Compensatory Islet Expansion During Insulin Resistance During the state of insulin resistance or over-weight, elevated plasma insulin levels (named hyperinsulinemia) has been found in polygenic mouse models showing insulin resistance and human subjects because of increasing of insulin secretion (Yalow and Berson, 1960; Polonsky et al., 1988; Brà ¼ning et al., 1997). It has been suggested later that both in rodents and people, this improved insulin secretion is apparently because of an expansion in ÃŽÂ ²-cell mass by either ÃŽÂ ²-cell proliferation (Steil et al., 2001) or ÃŽÂ ²-cell hypertrophy (Weir and Bonner-Weir, 2004). On the other hand, ÃŽÂ ²-cell failure because of different genetic or environmental variables, is known to cause declined plasma insulin levels in diabetics (Maclean and Ogilvie, 1955). It has been showed that lessened levels of insulin are frequently associated with a noteworthy loss of ÃŽÂ ²-cell mass because of ÃŽÂ ²-cell apoptosis (Butler et al., 2003; Rhodes, 2005). Other than the diabetes perspective, it has been demonstrated that matured ÃŽÂ ²-cells have long life-span and low proliferative rates at steady state. This is because of a potential limitation of the entry of matured ÃŽÂ ²-cells into cell cycle (Teta et al., 2005; Kushner, 2013). Other than this perception, later study suggested that adult ÃŽÂ ²-cells do have the ability to proliferate (Stolovich-Rain et al., 2012). In light of these findings on ÃŽÂ ²-cell proliferation, a few research groups have revealed various proteins essential for assisting ÃŽÂ ²-cell proliferation on knockout or transgenic mouse. Known cell cycle controllers including Cyclins D1 and D2, Cyclin subordinate kinase 4 (Cdk4), Cdk inhibitors (CKIs) such as Cip/Kip and INK4, transcription factors Retinoblastoma (Rb) and p53, have been proved on genetic mouse models as regulators of ÃŽÂ ²-cell proliferation and survival (Heit et al., 2006). Although transient high glucose has been considered as the result of insufficient insulin secretion or insulin resistance, it has also been revealed to promote the compensate ÃŽÂ ²-cell mass expansion (Bonner-Weir et al., 1989). This hypothesis was further supported recently by another observation, that it is the glucose metabolism, instead the glucose itself that triggers compensatory ÃŽÂ ²-cell proliferation in vivo (Porat et al., 2011). Some other attentions have been centered on the effect of activation of insulin/IRS2 pathway on driving ÃŽÂ ²-cell proliferation. The components of the pathway including IRS2 (Withers et al., 1998) and AKT (Bernal-Mizrachi et al., 2001) were demonstrated to be fundamental for ÃŽÂ ²-cell survival. Moreover, study has shown that the impact of insulin in ÃŽÂ ²-cell proliferation is even stronger when with hyperglycemia (Paris et al., 2003). It is notable that when in the state of severe insulin resistance, the pancreatic islets adjust t hemselves to meet the expanding requirement for producing and secretion more insulin by increasing their ÃŽÂ ²-cell mass (Weir and Bonner-Weir, 2004). Other than the involvement of protein coding genes, a few non-coding RNAs (ncRNAs), mostly microRNAs and long ncRNAs (lncRNAs), have been shown in the diabetogenes list, adding on to the complex genetic architecture of human diabetes. 2.1 The Brief History of MicroRNAs The most recent decade has seen huge attention regarding a new and special class of little ncRNAs such as microRNAs (miRNAs) in regulating the structure and function of ÃŽÂ ²-cells. With the first observation of a miRNA, lin-4 in C. elegans, researchers showed how a gene product encodes two little RNAs, instead of a protein. Besides, they demonstrated that these small RNAs binds to the compensatory sites at the 3 end of untranslated region (UTR) of lin-14, a development related heterochronic gene. This interaction is appeared to negatively regulate the expression of lin-14 by blocking its translation (Lee et al., 1993; Wightman et al., 1993), proposing miRNAs as negative regulators of gene expression. Even since the discovery of microRNAs, a lot of related research results have updated the mechanism of gene regulation to a novel level. miRNAs now are known as a group of small ncRNAs of ~21-22 nucleotides long that can complementary or non-complementary base-pairing the mRNA of protein coding genes, thus to regulate their expression at post-transcriptional level (Bartel, 2004 and 2009). Actually after the identification of lin-4, another miRNA called let-7 was revealed like lin-4 in both biogenesis and function level. Soon after, there starts a prevail in discovering new microRNASs mostly by high throughput sequencing technologies in research area and surprisingly, about 30,000 miRNA over about 200 species have been identified, which includes about 2,500 mature human miRNAs. (Kozomara and Griffiths-Jones, 2014). Many computational methods have also been produced to predict the potential targets of miRNAs based on the stable miRNA-target mRNA binding model (Lewis et al., 2003; Krek et al., 2005). 2.2 MicroRNA Induced Gene Silencing The intercellular gene silencing mechanism, termed as RNA silencing (RNAi) or post-transcriptional gene silencing (PTGS) is currently well known to be led by a group of small RNAs, for example, short interfering RNA (siRNA), piwi interacting RNA (piRNA), or the miRNAs. Basically, their working mechanisms are similar and the difference exist mostly in their biogenesis inside the cells (Ender and Meister, 2010). Mature miRNA producing has been through several steps: transcribed from DNA by RNA polymerase II, primary miRNA (pri-miRNA) has much longer sequence. Once transcribed, the pri-miRNA is further processed to precursor miRNA (pre-miRNA) about 60 nucleotides long by enzyme Dorsa and DGCR8 protein complex (Lee et al., 2003). Once pre-miRNAs are produced inside the nucleus, it will be export out of the nucleus by protein Exportin 5 to the cytoplasm in a Ran-GTP dependent manner (Lund et al., 2004). Another important enzyme, which is also critical for mouse development, Dicer would re cognize the pre-miRNA and process it to about 22 nucleotides long mature miRNA duplex form (Bernstein et al.,2003). Only one of stands of the duplex will be transported to miRNA-induced silencing complex (RISC) by Dicer, and the other strand, termed miRNA* is usually degraded in the end (Schwarz et al., 2003; Ender Meister, 2010). There are several components on the RISC, and one key protein component is AGO family. There are four well characterized members of AGO family in human AGO1, AGO2, AGO3 and AGO4. And AGO2 is expressed more often than other forms (Su et al., 2009; Wang et al., 2012). All the AGO proteins have the ability to slice mRNA because of their PAZ and PIWI cleavage domains under the guidance of miRNA sequence. Each miRNA has an important seed region, typically from 2nd-7th nucleotides, that could fully or partially bind to the mRNA 3 UTR sequence. The base pairing condition between the miRNA and target mRNA determines the target recognition and binding of miRISC, but also the fate of the mRNA- to be cleaved or to be repressed in translation. In animal system, the miRNA does not fully complementary bind to the 3 UTR of the target mRNA, through blocking the translation machinery, miRNA silencing the gene expression, without interference of the target stability. However, recent studies on the miR NA mediated gene silencing in mammalian cells reveals that miRNA may act through two step modes: at first, repressing the translation, then deadenylation and destabilization of target mRNA [27qualifier]. The deadenylation has been suggested to induce mRNA degradation [48] and translation inhibition is probably the requisite of mRNA degradation in mammalian cells [49].

Punk :: essays research papers

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Essay --

The novel "To Kill a Mockingbird" by Harper Lee is a simplistic view of life in the Deep South of America in the 1930s. An innocent but humorous stance in the story is through the eyes of Scout and Jem Finch. Scout is a young adolescent who is growing up with the controversy that surrounds her fathers lawsuit. Her father, Atticus Finch is a lawyer who is defending a black man, Tom Robinson, with the charge of raping a white girl. The lives of the characters are changed by racism and this is the force that develops during the course of the narrative. As previously mentioned, the point of view in this narrative is from Scout. Her upbringing has been respectful to the African-Americans in her society and she shows this with her relationship with her maid, Calpurnia. Other children her age have adopted their parents' racially prejudice views, causing her of many problems. Atticus's lawsuit seems to isolate his children and Scout is taunted with remarks in the playground. Her only retort is violence and Atticus, as an virtuous father, does not condone this behaviour either: "My fist...

Modern Art Essay

Some people consider modern arts not serious. In this regard, the question â€Å"Can we think of modern art as real art? † comes into existence. So to answer to this question we need to take for consideration what is art in general and what forms it has. There are a variety of arts, including visual arts and design, decorative arts, plastic arts and the performing arts. So, Art is defined as the expression or application of human creative skill and imagination. And artistic expression takes many forms: painting, drawing, printmaking, sculpture, music, literature, and architecture are the most widely recognized forms. To begin with, when we speak about modern art, in most cases we think about the field of fine arts. Because exactly fine arts underwent the visible changes for centuries. The number of styles of painting is enormous. Artistic works can be grouped by styles according to the common principles of creative thinking. Each epoch is characterized by a certain worldview; that is why throughout the centuries the ways of thinking about art was also changing. Fine arts faced such styles as Gothic, Renaissance, Baroque, Classicism, Realism, Impressionism and so on. Modern art refers to works produced during the period from 1870. Typically, modern artists rejected previous Renaissance-based traditions, in favour of new forms of artistic experimentation. They used new materials, new techniques of painting, and developed new theories about how art should reflect the perceived world. And nowadays there exists a great amount of styles of Modern art, for example: Abstract Expressionism, Pop Art, Performance Art, Dada Style, Neoplasticism, Surrealist Style, Postminimalism, Graffiti Art, etc. Concerning the question of modern art it’s quite difficult to judge, but nevertheless people are divided into two sides: pros and cons. First and foremost art implies the inseparability of form and content and of course talent. Famous artists affirmed that it is necessary to master the skill – the main component of the art. But many modern works have lack of this component. Modern art has no its purpose to create the artistic work, that is why modern artists are indifferent to internal connection of form and content. Therefore it is impossible without accompanying comment; for example the artist can grab a canvas, paint it in different colours and put some strokes on it, and this kind of painting can represent his happiness. Because it is often said that modern art can be done without any talent or efforts. After all real art needs to show emotion, beauty, and needs to be a window to the artist’s  emotions. Art is something that you see and must look deeply into to understand or find beauty in. Modern art is art that a 5 year old can do so it has too little effort to be considered art. Many people believe that such so called artists are supported by the mass media, that is why their works are sold for million dollars. Every person could take a brush and do smth like that, but no one would buy this daub. They say Leonardo was a great artist. Raphael was a great artist, but modern ones can’t be compared. So for the great majority of people modern art is not serious. On the other hand, who is anyone to judge what â€Å"real† art is? After all our understanding of art comes to nothing more than our school knowledge about periods of Renaissance, Classicism or Modern. Just because one doesn’t like or understand the art, doesn’t mean that the art is not real art. Besides, it should be noted that there is the term â€Å"contemporary art†, meaning the work of artists of the 90-ies of XX century. It represents the mirror of contemporary world. And often great

Feminist Contributions Essay

Feminism is a conflict theory which views society as male dominated and it seeks to describe, explain and change the position of women in society. Feminism is therefore a theory of women’s subordination and also a political movement. There are different types of feminism, which I will be evaluating in this essay; Liberal, Radical, Marxist, Black and Postmodernist feminist. Feminists criticise mainstream sociology for being ‘malestream’. By contrast, feminists examine society from the viewpoint of women an see their work as part of the struggle against women’s subordination. However, although all feminists oppose women’s subordination, there are disagreements among feminist’s theories about its causes and how to overcome them. Liberal feminists are concerned with the human and civil rights and freedoms of the individual, they believe that all human beings should have equal rights. In liberal feminism, the concept of society changing itself to adapt to women does not occur. Liberal feminists insist that all that is needed to change the status of women is to change existing laws that are unfavorable for women and that this will open up more opportunities for women to prove themselves as equal to the opposite sex, this is referred to as reformism. In addition, liberal feminists reject the idea that biological differences make women less competent or rational than me, nor are men biologically less emotional or nurturing than women are. Liberal feminist Oakley, distinguishes between sex and gender. She claims sex differences are seen as fixed, whereas gender differences vary between cultures and over time. Therefore what is considered a proper role for women in one society or at a specific time may be disapproved of or forbidden in another. According to liberal theorists, sexist attitudes and stereotypical beliefs about gender are culturally constructed and transmitted through socialisation. However, liberal feminism can be seen as a critique of the functionalist view of gender roles. For example, Parsons distinguishes between instrumental and expressive roles. In his view, instrumentals roles such as paid work are taken on by males, while expressive roles such as unpaid domestic labour and childbearing are taken on by females. On the other hand liberal feminism challenges this view. It argues that men and women are equally capable of  performing each roles. Radical feminists emerged in the early 1970s and its key concept is patriarchy. Radical feminists have three key arguments; patriarchy is universal as male domination of women is evident in all societies. Firestone argues that the origins of patriarchy lie in women’s biological capacity to bear and care for infants, as a result of this women become dependent on men. The second argument is that patriarchy is the primary and most fundamental form of social inequality and conflict. Thirdly, all men oppress women and all men benefit from patriarchy. Particularly from women’s unpaid domestic labour and from their sexual services. For radical feminists, patriarchal oppression occurs in the public sphere of work and politics and also in the private sphere of the family. According to radical feminists, patriarchal power is expressed through sexual or physical violence or the threat of it. As Brownmiller notes, fear of rape is a powerful deterrent against women going out alone at night. Likewise Rich argues that men force women into a narrow and unsatisfying ‘compulsory heterosexuality’, which becomes the only socially acceptable form of sexuality. Radical feminists believe that if women are to be free, personal and sexual relations must be transformed. They have suggested three strategies to achieve this. Firstly separatism, which is when women live apart from men and create a new culture of female independence. Feminist Greer argues for the creation of all-female or ‘matrilocal’ households as an alternative to the heterosexual family. The second strategy is consciousness raising, through sharing their experiences in women-only consciousness-raising groups, women come to see that other women face the same problems. The third strategy is political lesbianism; many radical feminists argue that heterosexual relationships are inevitably oppressive because they involve ‘sleeping with the enemy’ and that lesbianism is the only non-oppressive form of sexuality. Marxists have a key criticism of radical feminists. They argue that class is the primary form of inequality and that capitalism is the main cause and beneificiary of women’s oppression, not men. Another criticism comes from Anna Pollert, who argues that the concept of patriarchy is of little value in explaining women’s position because it involves a circular argument. In contrast, Marxist feminists dismiss liberal feminists’ view that women’s subordination is merely the product of stereotyping or outdate attitudes. Likewise they  also reject the radical feminist view that it is the result of patriarchal oppression by men. Marxists see women’s subordination as rooted in capitalism. This results from their primary role as unpaid homemakers, which presents them in dependent economic positions in the family. Their subordination performs four important functions of capitalism; women are a source of cheap, exploitable labour. As they are viewed as financially dependent on their male partners, they can be paid less by employers. Women are a reserve army of labour, that can be moved into the labour force during economic booms and out again at times of recession. Women produce the labour force through their unpaid domestic labour, by nurturing and socialising children to become the next generation of workers by maintaining and servicing the current generation of workers. Thirdly, women absorb anger that would otherwise be directed at capitalism. Ansley describes wives as ‘takers of shitâ €™ who soak up the frustration of their husbands, this explains male domestic violence against women.